Recombinant human YARS2 protein
YARS2, also known as tyrosyl-tRNA synthetase, catalyzes the attachment of tyrosine to tRNA (Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA (Tyr) . Recombinant human YARS2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Tyrosyl-tRNA synthetase mitochondrial precursor, Tyrosyl-tRNA synthetase, mitochondrial precursor, CGI-04, FLJ13995, MLASA2, MT-TYRRS, TYRRS
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
53.6 kDa (482aa)
Additionnal Information
References & Citations
Bonnefond L., et al. (2005) Biochemistry. 44:4805-4816; ; Riley L.G., et al. (2010) Am. J. Hum. Genet. 87:52-59
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_001035526
Uniprot Accession Number
Q9Y2Z4
Species
Human
AA Sequence
Frequently Asked Questions
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