Recombinant human YARS2 protein

YARS2, also known as tyrosyl-tRNA synthetase, catalyzes the attachment of tyrosine to tRNA (Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and then transferred to the acceptor end of tRNA (Tyr) . Recombinant human YARS2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Tyrosyl-tRNA synthetase mitochondrial precursor, Tyrosyl-tRNA synthetase, mitochondrial precursor, CGI-04, FLJ13995, MLASA2, MT-TYRRS, TYRRS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

53.6 kDa (482aa)

Additionnal Information

YARS2, Tyrosyl-tRNA synthetase mitochondrial precursor, Tyrosyl-tRNA synthetase, mitochondrial precursor, CGI-04, FLJ13995, MLASA2, MT-TYRRS, TYRRS, ATGP1554-10 µg, ATGP1554-20 µg, ATGP1554-50 µg, ATGP1554-100 µg, ATGP1554-250 µg, ATGP1554-500 µg, ATGP1554-1 mg, ATGP1554-10, ATGP1554-20, ATGP1554-50, ATGP1554-100, ATGP1554-250, ATGP1554-500, ATGP1554-1

References & Citations

Bonnefond L., et al. (2005) Biochemistry. 44:4805-4816; ; Riley L.G., et al. (2010) Am. J. Hum. Genet. 87:52-59

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_001035526

Uniprot Accession Number

Q9Y2Z4

Species

Human

AA Sequence

TLNLSVLLP LGLRKAHSGA QGLLAAQKAR GLFKDFFPET GTKIELPELF DRGTASFPQT IYCGFDPTAD SLHVGHLLAL LGLFHLQRAG HNVIALVGGA TARLGDPSGR TKEREALETE RVRANARALR LGLEALAANH QQLFTDGRSW GSFTVLDNSA WYQKQHLVDF LAAVGGHFRM GTLLSRQSVQ LRLKSPEGMS LAEFFYQVLQ AYDFYYLFQR YGCRVQLGGS DQLGNIMSGY EFINKLTGED VFGITVPLIT STTGAKLGKS AGNAVWLNRD KTSPFELYQF FVRQPDDSVE RYLKLFTFLP LPEIDHIMQL HVKEPERRGP QKRLAAEVTK LVHGREGLDS AKRCTQALYH SSIDALEVMS DQELKELFKE APFSEFFLDP GTSVLDTCRK ANAIPDGPRG YRMITEGGVS INHQQVTNPE SVLIVGQHIL KNGLSLLKIG KRNFYIIKWL QL