Recombinant human BRDG1/STAP1 protein

STAP1, also known as signal-transducing adaptor protein 1, appears to functions as a docking protein acting downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The protein is directly phosphorylated by Tec in vitro where it participates in a positive feedback loop, increasing Tec activity. Recombinant human STAP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Signal-transducing adaptor protein 1, BRDG1, STAP-1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

36.8 kDa (319aa) confirmed by MALDI-TOF

Additionnal Information

BRDG1, Signal-transducing adaptor protein 1, BRDG1, STAP-1, ATGP1552-10 µg, ATGP1552-20 µg, ATGP1552-50 µg, ATGP1552-100 µg, ATGP1552-250 µg, ATGP1552-500 µg, ATGP1552-1 mg, ATGP1552-10, ATGP1552-20, ATGP1552-50, ATGP1552-100, ATGP1552-250, ATGP1552-500, ATGP1552-1

References & Citations

Ohya K., et al. (1999) Proc. Natl. Acad. Sci. u.S.A. 96:11976-11981; ; Beausoleil S.A., et al. (2006) Nat. Biotechnol. 24:1285-1292

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Signal Transduction

NCBI Accession Number

NP_036240

Uniprot Accession Number

Q9ULZ2

Species

Human

AA Sequence

MMAKKP PKPAPRRIFQ ERLKITALPL YFEGFLLIKR SGYREYEHYW TELRGTTLFF YTDKKSIIYV DKLDIVDLTC LTEQNSTEKN CAKFTLVLPK EEVQLKTENT ESGEEWRGFI LTVTELSVPQ NVSLLPGQVI KLHEVLEREK KRRIETEQST SVEKEKEPTE DYVDVLNPMP ACFYTVSRKE ATEMLQKNPS LGNMILRPGS DSRNYSITIR QEIDIPRIKH YKVMSVGQNY TIELEKPVTL PNLFSVIDYF VKETRGNLRP FICSTDENTG QEPSMEGRSE KLKKNPHIA