Recombinant human GTF2E2 protein

GTF2E2, also known as TFIIE, is essential for transcription initiation by RNA polymerase II in conjunction with other general factors. TFIIE is a heterotetramer containing two subunits of relative molecular mass 57, 000 (TFIIE-alpha) and two of 34, 000 (TFIIE-beta) . TFIIE-beta is required in conjunction with TFIIE-alpha for transcription initiation. GTF2E2 recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase. Recombinant human GTF2E2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Transcription initiation factor IIE subunit beta, FE, TF2E2, TFIIE-B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

35.6 kDa (315aa) confirmed by MALDI-TOF

Additionnal Information

GTF2E2, Transcription initiation factor IIE subunit beta, FE, TF2E2, TFIIE-B, ATGP1547-10 µg, ATGP1547-20 µg, ATGP1547-50 µg, ATGP1547-100 µg, ATGP1547-250 µg, ATGP1547-500 µg, ATGP1547-1 mg, ATGP1547-10, ATGP1547-20, ATGP1547-50, ATGP1547-100, ATGP1547-250, ATGP1547-500, ATGP1547-1

References & Citations

Kang S.-W., et al. (2000) Genes Cells. 5:251-263; ; Liu L., et al. (2009) J. Biol. Chem. 284:5165-5174

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_002086

Uniprot Accession Number

P29084

Species

Human

AA Sequence

MDPSLL RERELFKKRA LSTPVVEKRS ASSESSSSSS KKKKTKVEHG GSSGSKQNSD HSNGSFNLKA LSGSSGYKFG VLAKIVNYMK TRHQRGDTHP LTLDEILDET QHLDIGLKQK QWLMTEALVN NPKIEVIDGK YAFKPKYNVR DKKALLRLLD QHDQRGLGGI LLEDIEEALP NSQKAVKALG DQILFVNRPD KKKILFFNDK SCQFSVDEEF QKLWRSVTVD SMDEEKIEEY LKRQGISSMQ ESGPKKVAPI QRRKKPASQK KRRFKTHNEH LAGVLKDYSD ITSSK