Recombinant human ERCC1 protein

ERCC1, also known as DNA excision repair protein ERCC-1, functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by uV light or formed by electrophilic compounds including cisplatin. Defects in ERCC1 are the cause of cerebro-oculo-facio-skeletal syndrome type 4 (COFS4) . Recombinant human ERCC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

DNA excision repair protein ERCC-1, COFS4, RAD10, uV20

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

32.5 kDa (297aa) confirmed by MALDI-TOF

Additionnal Information

ERCC1, DNA excision repair protein ERCC-1, COFS4, RAD10, uV20, ATGP1546-10 µg, ATGP1546-20 µg, ATGP1546-50 µg, ATGP1546-100 µg, ATGP1546-250 µg, ATGP1546-500 µg, ATGP1546-1 mg, ATGP1546-10, ATGP1546-20, ATGP1546-50, ATGP1546-100, ATGP1546-250, ATGP1546-500, ATGP1546-1

References & Citations

Van Duin M., et al. (1986) Cell. 44:913-923; ; Grimwood J., et al. (2004) Nature. 428:529-535

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Cancer

NCBI Accession Number

NP_001159521.1

Uniprot Accession Number

P07992

Species

Human

AA Sequence

MDPGKD KEGVPQPSGP PARKKFVIPL DEDEVPPGVA KPLFRSTQSL PTVDTSAQAA PQTYAEYAIS QPLEGAGATC PTGSEPLAGE TPNQALKPGA KSNSIIVSPR QRGNPVLKFV RNVPWEFGDV IPDYVLGQST CALFLSLRYH NLHPDYIHGR LQSLGKNFAL RVLLVQVDVK DPQQALKELA KMCILADCTL ILAWSPEEAG RYLETYKAYE QKPADLLMEK LEQDFVSRSL EQLIAASRED LALCPGLGPQ KARRLFDVLH EPFLKVP