Recombinant human CETN1 protein

Centrin, also known as CETN1, is an EF-hand type Ca2+-binding protein. It is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. CETN1 plays an important role in the determination of centrosome position and segregation, and in the process of microtubule severing. Recombinant human CETN1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Centrin, CEN1, CETN

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

22.1 kDa (196aa) confirmed by MALDI-TOF

Additionnal Information

CETN1, Centrin, CEN1, CETN, ATGP1544-10 µg, ATGP1544-20 µg, ATGP1544-50 µg, ATGP1544-100 µg, ATGP1544-250 µg, ATGP1544-500 µg, ATGP1544-1 mg, ATGP1544-10, ATGP1544-20, ATGP1544-50, ATGP1544-100, ATGP1544-250, ATGP1544-500, ATGP1544-1

References & Citations

LeDizet M., et al. (1998) Am J Physiol. 275:L1145-1156.; ; Araki M., et al. (2001) J Biol Chem. 276: 18665-18672.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_004057

Uniprot Accession Number

Q12798

Species

Human

AA Sequence

MASGFK KPSAASTGQK RKVAPKPELT EDQKQEVREA FDLFDVDGSG TIDAKELKVA MRALGFEPRK EEMKKMISEV DREGTGKISF NDFLAVMTQK MSEKDTKEEI LKAFRLFDDD ETGKISFKNL KRVANELGEN LTDEELQEMI DEADRDGDGE VNEEEFLRIM KKTSLY