Recombinant human PD-L1/B7-H1 protein (CD274)

CD274, also known as programmed cell death 1 ligand 1 (PDCD1L1), is involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. This protein interaction with PDCD1 inhibits T-cell proliferation and cytokine production. Recombinant human CD274 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Programmed cell death 1 ligand, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.9 kDa (245aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

PD-L1, B7-H1, Programmed cell death 1 ligand, B7-H, B7H1, PD-L1, PDCD1L1, PDCD1LG1, PDL1, ATGP1543-10 µg, ATGP1543-20 µg, ATGP1543-50 µg, ATGP1543-100 µg, ATGP1543-250 µg, ATGP1543-500 µg, ATGP1543-1 mg, ATGP1543-10, ATGP1543-20, ATGP1543-50, ATGP1543-100, ATGP1543-250, ATGP1543-500, ATGP1543-1

References & Citations

Dong H., et al. (1999) Nat. Med. 5:1365-1369; ; Freeman G.J., et al. (2000) J. Exp. Med. 192:1027-1034

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Scientific Category

Immunology

NCBI Accession Number

NP_054862

Uniprot Accession Number

Q9NZQ7

Species

Human

AA Sequence

FTVTV PKDLYVVEYG SNMTIECKFP VEKQLDLAAL IVYWEMEDKN IIQFVHGEED LKVQHSSYRQ RARLLKDQLS LGNAALQITD VKLQDAGVYR CMISYGGADY KRITVKVNAP YNKINQRILV VDPVTSEHEL TCQAEGYPKA EVIWTSSDHQ VLSGKTTTTN SKREEKLFNV TSTLRINTTT NEIFYCTFRR LDPEENHTAE LVIPELPLAH PPNER