Recombinant human PQBP1 protein

Polyglutamine binding protein 1, also known as PQBP1, is a transcription repressor that associates with polyglutamine tract-containing transcription regulators and causative genes for neurodegenerative disorders. PQBP-1 localizes to the nucleus and is present in neurons throughout the brain, with abundant levels in hippocampus, cerebellar cortex and olfactory bulb. PQBP-1 contains a WWP/WW domain that binds proline-rich motifs and a C2 domain that can influence Ca2+-dependent phospholipid signaling. Recombinant human PQBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Polyglutamine binding protein 1, MRX55, MRXS3, MRXS8, NPW38, RENS1, SHS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

33 kDa (289aa) confirmed by MALDI-TOF

Additionnal Information

PQBP1, Polyglutamine binding protein 1, MRX55, MRXS3, MRXS8, NPW38, RENS1, SHS, ATGP1540-10 µg, ATGP1540-20 µg, ATGP1540-50 µg, ATGP1540-100 µg, ATGP1540-250 µg, ATGP1540-500 µg, ATGP1540-1 mg, ATGP1540-10, ATGP1540-20, ATGP1540-50, ATGP1540-100, ATGP1540-250, ATGP1540-500, ATGP1540-1

References & Citations

Waragai M., et al. (1999) Hum Mol Genet. 8:977-987.; ; Waragai M C., et al. (2000) Biochem Biophys Res. 273: 592-595.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_005701.1

Uniprot Accession Number

O60828

Species

Human

AA Sequence

MPLPVA LQTRLAKRGI LKHLEPEPEE EIIAEDYDDD PVDYEATRLE GLPPSWYKVF DPSCGLPYYW NADTDLVSWL SPHDPNSVVT KSAKKLRSSN ADAEEKLDRS HDKSDRGHDK SDRSHEKLDR GHDKSDRGHD KSDRDRERGY DKVDRERERD RERDRDRGYD KADREEGKER RHHRREELAP YPKSKKAVSR KDEELDPMDP SSYSDAPRGT WSTGLPKRNE AKTGADTTAA GPLFQQRPYP SPGAVLRANA EASRTKQQD