Recombinant e.coli melA protein

MelA (alpha-galactosidase) belongs to glycosyl hydrolase 4 family. Alpha-galactosidases catalyse the hydrolysis of saccharides containing o-1, 6, -galactoside linkages. The three alpha-galactosidases catalyse the same reaction, but are localized in different cellular compartments: The E. coli enzyme is cytoplasmic protein and the human enzyme and the yeast enzyme is secretory proteins. Therefore, although the active enzyme from all three species has nearly the same molecular weight, structural similarities, as well as differences, are expected. Recombinant E. coil melA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Alpha-galactosidase, ECK4112, JW4080, mel-7

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

53 kDa (474aa)

Additionnal Information

MelA, Alpha-galactosidase, ECK4112, JW4080, mel-7, ATGP1535-10 µg, ATGP1535-20 µg, ATGP1535-50 µg, ATGP1535-100 µg, ATGP1535-250 µg, ATGP1535-500 µg, ATGP1535-1 mg, ATGP1535-10, ATGP1535-20, ATGP1535-50, ATGP1535-100, ATGP1535-250, ATGP1535-500, ATGP1535-1

References & Citations

Naumov G., et al. (1990) Mol Gen Genet. 224 (1) :119-28.; ; Naumov G., et al. (1995) Yeast. 30 (5) : 481-3.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_418543

Uniprot Accession Number

P06720

Species

E.coli

AA Sequence

MMSAPKI TFIGAGSTIF VKNILGDVFH REALKTAHIA LMDIDPTRLE ESHIVVRKLM DSAGASGKIT CHTQQKEALE DADFVVVAFQ IGGYEPCTVT DFEVCKRHGL EQTIADTLGP GGIMRALRTI PHLWQICEDM TEVCPDATML NYVNPMAMNT WAMYARYPHI KQVGLCHSVQ GTAEELARDL NIDPATLRYR CAGINHMAFY LELERKTADG SYVNLYPELL AAYEAGQAPK PNIHGNTRCQ NIVRYEMFKK LGYFVTESSE HFAEYTPWFI KPGREDLIER YKVPLDEYPK RCVEQLANWH KELEEYKKAS RIDIKPSREY ASTIMNAIWT GEPSVIYGNV RNDGLIDNLP QGCCVEVACL VDANGIQPTK VGTLPSHLAA LMQTNINVQT LLTEAILTEN RDRVYHAAMM DPHTAAVLGI DEIYALVDDL IAAHGDWLPG WLHR