Recombinant e.coli melA protein
MelA (alpha-galactosidase) belongs to glycosyl hydrolase 4 family. Alpha-galactosidases catalyse the hydrolysis of saccharides containing o-1, 6, -galactoside linkages. The three alpha-galactosidases catalyse the same reaction, but are localized in different cellular compartments: The E. coli enzyme is cytoplasmic protein and the human enzyme and the yeast enzyme is secretory proteins. Therefore, although the active enzyme from all three species has nearly the same molecular weight, structural similarities, as well as differences, are expected. Recombinant E. coil melA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Alpha-galactosidase, ECK4112, JW4080, mel-7
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
53 kDa (474aa)
Additionnal Information
References & Citations
Naumov G., et al. (1990) Mol Gen Genet. 224 (1) :119-28.; ; Naumov G., et al. (1995) Yeast. 30 (5) : 481-3.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_418543
Uniprot Accession Number
P06720
Species
E.coli
AA Sequence
Frequently Asked Questions
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