Recombinant human TCEA1 protein

TCEA1 (Transcription elongation factor A protein 1) belongs to the TFS-II family. TCEA1 binds to Pol II and functions to cleave the nascent transcript, thereby unlocking the complex and allowing transcription to continue. Localized to the nucleus, TCEA1 contains three independently-folding domains, all of which are necessary for proper binding to Pol II. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Recombinant human TCEA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Transcription elongation factor A protein 1, GTF2S, SII, TCEA, TF2S, TFIIS

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

36.5 kDa (325aa) confirmed by MALDI-TOF

Additionnal Information

TCEA1, Transcription elongation factor A protein 1, GTF2S, SII, TCEA, TF2S, TFIIS, ATGP1534-10 µg, ATGP1534-20 µg, ATGP1534-50 µg, ATGP1534-100 µg, ATGP1534-250 µg, ATGP1534-500 µg, ATGP1534-1 mg, ATGP1534-10, ATGP1534-20, ATGP1534-50, ATGP1534-100, ATGP1534-250, ATGP1534-500, ATGP1534-1

References & Citations

Kulish, D. et al. (2001) Mol. Cell. Biol. 21: 4162-4168.; ; Park H, et al. (1994) Gene 139 (2) : 263-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol, 1mM DTT, 50 mM NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_006747

Uniprot Accession Number

P23193

Species

Human

AA Sequence

HMEDEVV RFAKKMDKMV QKKNAAGALD LLKELKNIPM TLELLQSTRI GMSVNAIRKQ STDEEVTSLA KSLIKSWKKL LDGPSTEKDL DEKKKEPAIT SQNSPEAREE STSSGNVSNR KDETNARDTY VSSFPRAPST SDSVRLKCRE MLAAALRTGD DYIAIGADEE ELGSQIEEAI YQEIRNTDMK YKNRVRSRIS NLKDAKNPNL RKNVLCGNIP PDLFARMTAE EMASDELKEM RKNLTKEAIR EHQMAKTGGT QTDLFTCGKC KKKNCTYTQV QTRSADEPMT TFVVCNECGN RWKFC