Recombinant human TCEA1 protein
TCEA1 (Transcription elongation factor A protein 1) belongs to the TFS-II family. TCEA1 binds to Pol II and functions to cleave the nascent transcript, thereby unlocking the complex and allowing transcription to continue. Localized to the nucleus, TCEA1 contains three independently-folding domains, all of which are necessary for proper binding to Pol II. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. Recombinant human TCEA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Transcription elongation factor A protein 1, GTF2S, SII, TCEA, TF2S, TFIIS
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
36.5 kDa (325aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Kulish, D. et al. (2001) Mol. Cell. Biol. 21: 4162-4168.; ; Park H, et al. (1994) Gene 139 (2) : 263-7.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol, 1mM DTT, 50 mM NaCl
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_006747
Uniprot Accession Number
P23193
Species
Human
AA Sequence
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