Recombinant human Cystatin S protein
CST4 (Cystatin-S) belongs to the cystatin family. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. Recombinant human CST4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Cystatin S
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
16.8 kDa (145aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Saitoh E, et al. (1992) Biomed Biochim Acta 50 (4-6) : 599-605.; ; Ryan C.M., et al. (2010) J. Am. Soc. Mass Spectrom. 21:908-917
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl
Scientific Category
Immunology
NCBI Accession Number
NP_001890
Uniprot Accession Number
P01036
Species
Human
AA Sequence
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