Recombinant human Cystatin S protein

CST4 (Cystatin-S) belongs to the cystatin family. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. This protein strongly inhibits papain and ficin, partially inhibits stem bromelain and bovine cathepsin C, but does not inhibit porcine cathepsin B or clostripain. Papain is inhibited non-competitively. Recombinant human CST4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Cystatin S

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

16.8 kDa (145aa) confirmed by MALDI-TOF

Additionnal Information

Cystatin S, cystatin S, ATGP1531-10 µg, ATGP1531-20 µg, ATGP1531-50 µg, ATGP1531-100 µg, ATGP1531-250 µg, ATGP1531-500 µg, ATGP1531-1 mg, ATGP1531-10, ATGP1531-20, ATGP1531-50, ATGP1531-100, ATGP1531-250, ATGP1531-500, ATGP1531-1

References & Citations

Saitoh E, et al. (1992) Biomed Biochim Acta 50 (4-6) : 599-605.; ; Ryan C.M., et al. (2010) J. Am. Soc. Mass Spectrom. 21:908-917

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Scientific Category

Immunology

NCBI Accession Number

NP_001890

Uniprot Accession Number

P01036

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMSSSKEE NRIIPGGIYD ADLNDEWVQR ALHFAISEYN KATEDEYYRR PLQVLRAREQ TFGGVNYFFD VEVGRTICTK SQPNLDTCAF HEQPELQKKQ LCSFEIYEVP WEDRMSLVNS RCQEA