Recombinant human BCAT2 protein

BCAT2 belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family. This protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. It may also function as a transporter of branched chain alpha-keto acids. Recombinant human BCAT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Branched chain amino-acid aminotransferase 2 mitochondrial, Branched chain amino-acid aminotransferase 2, mitochondrial, BCAM, BCATM, BCT2, PP18, ECA40, Placental protein 18

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

43.9 kDa (390aa) confirmed by MALDI-TOF

Additionnal Information

BCAT2, Branched chain amino-acid aminotransferase 2 mitochondrial, Branched chain amino-acid aminotransferase 2, mitochondrial, BCAM, BCATM, BCT2, PP18, ATGP1523-10 µg, ATGP1523-20 µg, ATGP1523-50 µg, ATGP1523-100 µg, ATGP1523-250 µg, ATGP1523-500 µg, ATGP1523-1 mg, ATGP1523-10, ATGP1523-20, ATGP1523-50, ATGP1523-100, ATGP1523-250, ATGP1523-500, ATGP1523-1

References & Citations

Coles SJ. et al. (2009) Biochemistry Jan 27; ;48 (3) :645-56.; ; Yennawar N.H.et al. (2007) J. Biol. Chem. 281:39660-39671

Other References

Günther J, et al. BAY-069, a Novel (Trifluoromethyl) pyrimidinedione-Based BCAT1/2 Inhibitor and Chemical Probe. (J Med Chem. 2022) {https://pubmed.ncbi.nlm.nih.gov/36261130/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol 0.2M NaCl, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_001181

Uniprot Accession Number

O15382

Species

Human

AA Sequence

ASSSF KAADLQLEMT QKPHKKPGPG EPLVFGKTFT DHMLMVEWND KGWGQPRIQP FQNLTLHPAS SSLHYSLQLF EGMKAFKGKD QQVRLFRPWL NMDRMLRSAM RLCLPSFDKL ELLECIRRLI EVDKDWVPDA AGTSLYVRPV LIGNEPSLGV SQPTRALLFV ILCPVGAYFP GGSVTPVSLL ADPAFIRAWV GGVGNYKLGG NYGPTVLVQQ EALKRGCEQV LWLYGPDHQL TEVGTMNIFV YWTHEDGVLE LVTPPLNGVI LPGVVRQSLL DMAQTWGEFR VVERTITMKQ LLRALEEGRV REVFGSGTAC QVCPVHRILY KDRNLHIPTM ENGPELILRF QKELKEIQYG IRAHEWMFPV