Recombinant human MEK2 protein

MAP2K2, also known as MEK2, belongs to the STE grouping of kinases. Both MEK2 and the related MEK1 are dualspecificity kinases, phosphorylating and activating the mitogen-activated protein kinases ERK1 and ERK2 at T and Y positions within the phosphoacceptor sequence T-E-Y. MAP2K2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx. Recombinant human MAP2K2 proetin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Mitogen-activated protein kinase kinase 2, MAP kinase kinase 2, MAPKK2, MAPK/ERK kinase 2, MEK2, MKK2, PRKMK2, ERK activator kinase 2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

46.9 kDa (424aa) confirmed by MALDI-TOF

Additionnal Information

PRKMK-2, PRKMK2, PRKMK 2, MKK-2, MKK2, MKK 2, Mitogen-activated protein kinase kinase 2, Mitogen activated protein kinase kinase 2, MEK-2, MEK2, MEK 2, MAPKK-2, MAPKK2, MAPKK 2, MAPK/ERK kinase 2, MAP2K2, MAP kinase kinase 2, ERK activator kinase 2, Dual specificity mitogen-activated protein kinase kinase 2, Dual specificity mitogen activated protein kinase kinase 2, CFC4, ATGP1520-10 µg, ATGP1520-20 µg, ATGP1520-50 µg, ATGP1520-100 µg, ATGP1520-250 µg, ATGP1520-500 µg, ATGP1520-1 mg, ATGP1520-010, ATGP1520-020, ATGP1520-050, ATGP1520-100, ATGP1520-250, ATGP1520-500, ATGP1520-01M

References & Citations

Crews C M., et al. (1992) Science. 258:478-480.; ; Rosen L B., et al. (1994) Neuron. 12: 1207-1221.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_109587

Uniprot Accession Number

P36507

Species

Human

AA Sequence

MLARRK PVLPALTINP TIAEGPSPTS EGASEANLVD LQKKLEELEL DEQQKKRLEA FLTQKAKVGE LKDDDFERIS ELGAGNGGVV TKVQHRPSGL IMARKLIHLE IKPAIRNQII RELQVLHECN SPYIVGFYGA FYSDGEISIC MEHMDGGSLD QVLKEAKRIP EEILGKVSIA VLRGLAYLRE KHQIMHRDVK PSNILVNSRG EIKLCDFGVS GQLIDSMANS FVGTRSYMAP ERLQGTHYSV QSDIWSMGLS LVELAVGRYP IPPPDAKELE AIFGRPVVDG EEGEPHSISP RPRPPGRPVS GHGMDSRPAM AIFELLDYIV NEPPPKLPNG VFTPDFQEFV NKCLIKNPAE RADLKMLTNH TFIKRSEVEE VDFAGWLCKT LRLNQPGTPT RTAV