Recombinant mouse IgG2A protein
Immunoglobulin G (IgG) is antibody molecules. Each IgG is composed of four peptide chains -two heavy chains gamma and two light chains. Each IgG has two antigen binding sites. IgG antibodies are involved in predominantly the secondary immune response. The presence of specific IgG, in general, corresponds to maturation of the antibody response. It also plays an important role in Antibody-dependent cell-mediated cytotoxicity (ADCC) and Intracellular antibody-mediated proteolysis, in which it binds to TRIM21 (the receptor with greatest affinity to IgG in humans) in order to direct marked virions to the proteasome in the cytosol. Recombinant mouse IgG protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Immunoglobulin heavy chain constant region gamma 2a, Immunoglobulin G
Expression System
Baculovirus
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
27.5 kDa (242aa)
Additionnal Information
References & Citations
Mallery DL, et al. (2010) Proc. Natl. Acad. Sci. u.S.A. 107 (46) : 19985-19990.; ; Stadlmann J, et al. (2008) Proteomics 8 (14) : 2858-2871.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
CAC20702
Uniprot Accession Number
P01863
Species
Mouse
AA Sequence
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