Recombinant human VPS20/CHMP6 protein

CHMP6, as known as charged multivesicular body protein 6, belongs to the SNF7 family. This Protein is core component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Recombinant human CHMP6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Charged multivesicular body protein 6, VPS20, Chromatin-modifying protein 6, Vacuolar protein sorting-associated protein 20, hVps20

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

26 kDa (225aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

CHMP6, charged multivesicular body protein 6, VPS20, ATGP1511-10 µg, ATGP1511-20 µg, ATGP1511-50 µg, ATGP1511-100 µg, ATGP1511-250 µg, ATGP1511-500 µg, ATGP1511-1 mg, ATGP1511-10, ATGP1511-20, ATGP1511-50, ATGP1511-100, ATGP1511-250, ATGP1511-500, ATGP1511-1

References & Citations

Yorikawa C., et al. (2005) Biochem. J. 387:17-26; ; Howard TL, et al. (2001) J Cell Sci 114 (Pt 13) : 2395-404.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_078867

Uniprot Accession Number

Q96FZ7

Species

Human

AA Sequence

MGNLFG RKKQSRVTEQ DKAILQLKQQ RDKLRQYQKR IAQQLERERA LARQLLRDGR KERAKLLLKK KRYQEQLLDR TENQISSLEA MVQSIEFTQI EMKVMEGLQF GNECLNKMHQ VMSIEEVERI LDETQEAVEY QRQIDELLAG SFTQEDEDAI LEELSAITQE QIELPEVPSE PLPEKIPENV PVKARPRQAE LVAAS