Recombinant human UBA5 protein

UBA5 (ubiquitin-like modifier activating enzyme 5) belongs to the ubiquitin-activating E1 family and uBA5 subfamily. ubiquitin and ubiquitin-like proteins are known to be covalently conjugated to a variety of cellular substrates via a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) plays a key role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. uBA5 had been proved to activate an ubiquitin-like protein, ubiquitin-fold modifier 1 (ufm1), by forming a high-energy thioester bond. It localizes mainly in cytoplasm, while it mainly localizes to the nucleus in presence of SuMO2. Recombinant human uBA5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Ubiquitin-like modifier-activating enzyme 5, THIFP1, uBE1DC1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

47.4 kDa (428aa) confirmed by MALDI-TOF

Additionnal Information

UFM1-activating enzyme, UFM1 Activating Enzyme, Ubiquitin-like modifier-activating enzyme 5, Ubiquitin-activating enzyme E1 domain-containing protein 1, uBE1DC1, UBA5, THIFP1, Ubiquitin like modifier-activating enzyme 5, Ubiquitin activating enzyme E1 domain containing protein 1, Ubiquitin like modifier activating enzyme 5, ATGP1507-10 µg, ATGP1507-20 µg, ATGP1507-50 µg, ATGP1507-100 µg, ATGP1507-250 µg, ATGP1507-500 µg, ATGP1507-1 mg, ATGP1507-010, ATGP1507-020, ATGP1507-050, ATGP1507-100, ATGP1507-250, ATGP1507-500, ATGP1507-01M

References & Citations

Komatsu M., et al. (2004) EMBO J. 23:1977-1986; ; Zheng M., et al. (2008) J. Cell. Biochem. 104:2324-2334

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

Scientific Category

Ubiquitination

NCBI Accession Number

NP_079094

Uniprot Accession Number

Q9GZZ9

Species

Human

AA Sequence

MAESVE RLQQRVQELE RELAQERSLQ VPRSGDGGGG RVRIEKMSSE VVDSNPYSRL MALKRMGIVS DYEKIRTFAV AIVGVGGVGS VTAEMLTRCG IGKLLLFDYD KVELANMNRL FFQPHQAGLS KVQAAEHTLR NINPDVLFEV HNYNITTVEN FQHFMDRISN GGLEEGKPVD LVLSCVDNFE ARMTINTACN ELGQTWMESG VSENAVSGHI QLIIPGESAC FACAPPLVVA ANIDEKTLKR EGVCAASLPT TMGVVAGILV QNVLKFLLNF GTVSFYLGYN AMQDFFPTMS MKPNPQCDDR NCRKQQEEYK KKVAALPKQE VIQEEEEIIH EDNEWGIELV SEVSEEELKN FSGPVPDLPE GITVAYTIPK KQEDSVTELT VEDSGESLED LMAKMKNM