Recombinant human Endophilin A1/SH3GL2 protein

SH3GL2 (SH3-domain GRB2-like 2), also known as endophilin-A1, belongs to the endophilin family. It contains a BAR domain and a SH3 domain. Members of the BAR domain protein superfamily are essential elements of cellular traffic. Endophilins are among the best studied BAR domain proteins. They have a prominent function in synaptic vesicle endocytosis (SVE), receptor trafficking and apoptosis, and in other processes that require remodeling of the membrane structure. SH3GL2 a novel tumor suppressor gene in laryngeal squamous cell carcinoma (LSCC), induces apoptosis of tumor cells by regulating intra-cellular signal transduction networks. Recombinant human SH3GL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Endophilin-A1, CNSA2, EEN-B1, SH3D2A, SH3P4

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

42.5 kDa (376aa) confirmed by MALDI-TOF

Additionnal Information

Endophilin A1, SH3GL2, Endophilin-A1, CNSA2, EEN-B1, SH3D2A, SH3P4, ATGP1506-10 µg, ATGP1506-20 µg, ATGP1506-50 µg, ATGP1506-100 µg, ATGP1506-250 µg, ATGP1506-500 µg, ATGP1506-1 mg, ATGP1506-10, ATGP1506-20, ATGP1506-50, ATGP1506-100, ATGP1506-250, ATGP1506-500, ATGP1506-1

References & Citations

Shang C, Guo Y, Fu S, Fu W, Sun K.; ; Med Sci Monit. 2010 Jun; ;16 (6) :BR168-73.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Scientific Category

Cancer

NCBI Accession Number

NP_003017

Uniprot Accession Number

Q99962

Species

Human

AA Sequence

MSVAGL KKQFHKATQK VSEKVGGAEG TKLDDDFKEM ERKVDVTSRA VMEIMTKTIE YLQPNPASRA KLSMINTMSK IRGQEKGPGY PQAEALLAEA MLKFGRELGD DCNFGPALGE VGEAMRELSE VKDSLDIEVK QNFIDPLQNL HDKDLREIQH HLKKLEGRRL DFDYKKKRQG KIPDEELRQA LEKFDESKEI AESSMFNLLE MDIEQVSQLS ALVQAQLEYH KQAVQILQQV TVRLEERIRQ ASSQPRREYQ PKPRMSLEFP TGDSTQPNGG LSHTGTPKPS GVQMDQPCCR ALYDFEPENE GELGFKEGDI ITLTNQIDEN WYEGMLHGHS GFFPINYVEI LVALPH