Recombinant human PTGR2 protein

PTGR2 belongs to the medium-chain dehydrogenase/reductase superfamily. PTGR2 catalyzes an NADPH-dependent reduction of the conjugated alpha, beta-unsaturated double bond of 15-keto-PGE (2), a key step in terminal inactivation of prostaglandins and suppression of PPARgamma-mediated adipocyte differentiation. Selective inhibition of PTGR2 may contribute to the improvement of insulin sensitivity with fewer side effects. Recombinant human PTGR2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Prostaglandin reductase 2, PGR2, ZADH1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

41 kDa (375aa) confirmed by MALDI-TOF

Additionnal Information

PTGR2, Prostaglandin reductase 2, PGR2, ZADH1, ATGP1505-10 µg, ATGP1505-20 µg, ATGP1505-50 µg, ATGP1505-100 µg, ATGP1505-250 µg, ATGP1505-500 µg, ATGP1505-1 mg, ATGP1505-10, ATGP1505-20, ATGP1505-50, ATGP1505-100, ATGP1505-250, ATGP1505-500, ATGP1505-1

References & Citations

Wu Y.H., et al. (2008) Structure 16:1714-1723; ; Auld, D.S. et al. (2008) Cell. Mol. Life Sci. 65: 3961-3970.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Scientific Category

Redox Proteins

NCBI Accession Number

NP_689657.1

Uniprot Accession Number

Q8N8N7

Species

Human

AA Sequence

MIVQRV VLNSRPGKNG NPVAENFRME EVYLPDNINE GQVQVRTLYL SVDPYMRCRM NEDTGTDYIT PWQLSQVVDG GGIGIIEESK HTNLTKGDFV TSFYWPWQTK VILDGNSLEK VDPQLVDGHL SYFLGAIGMP GLTSLIGIQE KGHITAGSNK TMVVSGAAGA CGSVAGQIGH FLGCSRVVGI CGTHEKCILL TSELGFDAAI NYKKDNVAEQ LRESCPAGVD VYFDNVGGNI SDTVISQMNE NSHIILCGQI SQYNKDVPYP PPLSPAIEAI QKERNITRER FLVLNYKDKF EPGILQLSQW FKEGKLKIKE TVINGLENMG AAFQSMMTGG NIGKQIVCIS EEISL