Recombinant e.coli pykF protein

PyrF (pyruvate kinase) belongs to the pyruvate kinase family. pykF is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. This process also requires a Magnesium ion. This step is the final one in the glycolytic pathway, which produces pyruvate molecules, the final product of aerobic glycolysis. Recombinant E. coli pykF protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.