Recombinant human TRDMT1 protein
TRDMT1 (tRNA aspartic acid methyltransferase 1), also known as DNMT2, specifically methylates cytosine 38 in the anticodon loop of tRNA (Asp) . Recombinant human TRDMT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
TRNA (cytosine (38) -C (5) ) -methyltransferase, M.HsaIIP, PuMet, RNMT1, DNMT2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
47.1 kDa (415aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Okano M., et al. (1998) Nucleic Acids Res. 26:2536-2540; ; Franchina M., et al. (2001) Int. J. Biochem. Cell Biol. 33:1104-1115
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
AAH47733
Uniprot Accession Number
O14717
Species
Human
AA Sequence
Frequently Asked Questions
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