Recombinant human TRDMT1 protein

TRDMT1 (tRNA aspartic acid methyltransferase 1), also known as DNMT2, specifically methylates cytosine 38 in the anticodon loop of tRNA (Asp) . Recombinant human TRDMT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

TRNA (cytosine (38) -C (5) ) -methyltransferase, M.HsaIIP, PuMet, RNMT1, DNMT2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

47.1 kDa (415aa) confirmed by MALDI-TOF

Additionnal Information

TRDMT1, tRNA (cytosine (38) -C (5) ) -methyltransferase, M.HsaIIP, PuMet, RNMT1, DNMT2, ATGP1495-10 µg, ATGP1495-20 µg, ATGP1495-50 µg, ATGP1495-100 µg, ATGP1495-250 µg, ATGP1495-500 µg, ATGP1495-1 mg, ATGP1495-10, ATGP1495-20, ATGP1495-50, ATGP1495-100, ATGP1495-250, ATGP1495-500, ATGP1495-1

References & Citations

Okano M., et al. (1998) Nucleic Acids Res. 26:2536-2540; ; Franchina M., et al. (2001) Int. J. Biochem. Cell Biol. 33:1104-1115

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

AAH47733

Uniprot Accession Number

O14717

Species

Human

AA Sequence

MEPLRV LELYSGVGGM HHALRESCIP AQVVAAIDVN TVANEVYKYN FPHTQLLAKT IEGITLEEFD RLSFDMILMS PPCQPFTRIG RQGDMTDSRT NSFLYILDIL PRLQKLPKYI LLENVKGFEV SSTRDLLIQT IENCGFQYQE FLLSPTSLGI PNSRLRYFLI AKLQSEPLPF QAPGQVLMEF PKIESVHPQK YAMDVENKIQ EKNVEPNISF DGSIQCSGKD AILFKLETAE EIHRKNQQDS DLSVKMLKDF LEDDTDVNQY LLPPKSLLRY ALLLDIVQPT CRRSVCFTKG YGSYIEGTGS VLQTAEDVQV ENIYKSLTNL SQEEQITKLL ILKLRYFTPK EIANLLGFPP EFGFPEKITV KQRYRLLGNS LNVHVVAKLI KILYE