Recombinant human IFN-gamma/IFNG protein

IFNG, also known as interferon gamma, is secreted by lymphocytes stimulated by mitogen and is involved in the differentiation, maturation, and proliferation of hematopoietic cells. Natural human interferon-gamma is composed of 143 amino acid residues without cysteine residues and is glycosylated. Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161. Recombinant human IFNG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Interferon gamma, Immune interferon, IFG, IFI, IMD69

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

18.5 kDa (159aa) confirmed by MALDI-TOF

Additionnal Information

IFN-g, Interferon gamma, IFG, IFI, ATGP1493-10 µg, ATGP1493-20 µg, ATGP1493-50 µg, ATGP1493-100 µg, ATGP1493-250 µg, ATGP1493-500 µg, ATGP1493-1 mg, ATGP1493-10, ATGP1493-20, ATGP1493-50, ATGP1493-100, ATGP1493-250, ATGP1493-500, ATGP1493-1

References & Citations

Sen GC, Lengyel P (1992) J. Biol. Chem. 267 (8) :5017-5020; ; Tsutomu Arakawa, Yeuh-rong Hsu (1987) Biochemistry. 26:5428-5432; ; Emst Rinderknecht, et al. (1984) J. Biol. Chem. 259 (11) :6790-6797

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000610

Uniprot Accession Number

P01579

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFRG