Recombinant human HAUS1 protein

HAuS1, also known as HAuS augmin-like complex subunit 1, is 1 of 8 subunits of the 390-kD human augmin complex, or HAuS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb augmentare, meaning to increase. The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly. Recombinant human HAuS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

HAuS augmin-like complex subunit 1, CCDC5, HEI-C, HEIC, HsT1461, Coiled-coil domain containing 5, Enhancer of invasion-cluster

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

34.4 kDa (302aa) confirmed by MALDI-TOF

Additionnal Information

HAuS1, HAuS augmin-like complex subunit 1, CCDC5, HEI-C, HEIC, HsT1461, ATGP1491-10 µg, ATGP1491-20 µg, ATGP1491-50 µg, ATGP1491-100 µg, ATGP1491-250 µg, ATGP1491-500 µg, ATGP1491-1 mg, ATGP1491-10, ATGP1491-20, ATGP1491-50, ATGP1491-100, ATGP1491-250, ATGP1491-500, ATGP1491-1

References & Citations

Einarson M.B., et al. (2004) Mol. Cell. Biol. 24:3957-3971; ; Lawo S., et al. (2009) Curr. Biol. 19:816-826

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl, 2mM DTT

Scientific Category

Cell Cycle

NCBI Accession Number

NP_612452

Uniprot Accession Number

Q96CS2

Species

Human

AA Sequence

MEPQEE RETQVAAWLK KIFGDHPIPQ YEVNPRTTEI LHHLSERNRV RDRDVYLVIE DLKQKASEYE SEAKYLQDLL MESVNFSPAN LSSTGSRYLN ALVDSAVALE TKDTSLASFI PAVNDLTSDL FRTKSKSEEI KIELEKLEKN LTATLVLEKC LQEDVKKAEL HLSTERAKVD NRRQNMDFLK AKSEEFRFGI KAAEEQLSAR GMDASLSHQS LVALSEKLAR LKQQTIPLKK KLESYLDLMP NPSLAQVKIE EAKRELDSIE AELTRRVDMM EL