Recombinant M. tuberculosis Antigen 85A protein
Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C) . The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord factor (trehalose 6, 6'-dimycolate, TDM) is essential for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A. Recombinant Mycobacterium tuberculosis Antigen 85A protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
Baculovirus
Antigen Species
Mycobacterium tuberculosis
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
32.8 kDa (305aa)
Additionnal Information
References & Citations
Elamin AA., et al. (2009) J Microbiol Methods. 79 (3) :358-63.; ; Jeon BY., et al. (2011) Microbes Infect. 13 (3) :284-90.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol
Scientific Category
Other
NCBI Accession Number
NP_218321.1
Uniprot Accession Number
P9WQP3
Species
Mycobacterium tuberculosis
AA Sequence
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