Recombinant M. tuberculosis Antigen 85A protein

Antigen 85A, belong to the antigen 85 complex (Antigen 85A, B, C) . The enzymes of the antigen 85 complex possess mycolyltransferase activity and catalyze the synthesis of the most abundant glycolipid of the mycobacterial cell wall, the cord factor. The cord factor (trehalose 6, 6'-dimycolate, TDM) is essential for the integrity of the mycobacterial cell wall and pathogenesis of the bacillus. TDM is synthesized from two molecules of trehalose-6'-monomycolate (TMM) by Antigen 85A. Recombinant Mycobacterium tuberculosis Antigen 85A protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Antigen 85 complex A, Mycolytransferase 85A, Fibronectin-binding protein A, TB Ab85A, TBab85A, Ag85A, DGAT, Diacylglycerol acyltransferase/mycolyltransferase Ag85A, Acyl-CoA diacylglycerol acyltransferase, fbpA, Rv3804c

Expression System

Baculovirus

Antigen Species

Mycobacterium tuberculosis

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

32.8 kDa (305aa)

Additionnal Information

Antigen85A, Antigen 85 complex A, Mycolytransferase 85A, Fibronectin-binding protein A, TB Ab85A, TBab85A, ATGP1490-10 µg, ATGP1490-20 µg, ATGP1490-50 µg, ATGP1490-100 µg, ATGP1490-250 µg, ATGP1490-500 µg, ATGP1490-1 mg, ATGP1490-10, ATGP1490-20, ATGP1490-50, ATGP1490-100, ATGP1490-250, ATGP1490-500, ATGP1490-1

References & Citations

Elamin AA., et al. (2009) J Microbiol Methods. 79 (3) :358-63.; ; Jeon BY., et al. (2011) Microbes Infect. 13 (3) :284-90.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol

Scientific Category

Other

NCBI Accession Number

NP_218321.1

Uniprot Accession Number

P9WQP3

Species

Mycobacterium tuberculosis

AA Sequence

FSRPGL PVEYLQVPSP SMGRDIKVQF QSGGANSPAL YLLDGLRAQD DFSGWDINTP AFEWYDQSGL SVVMPVGGQS SFYSDWYQPA CGKAGCQTYK WETFLTSELP GWLQANRHVK PTGSAVVGLS MAASSALTLA IYHPQQFVYA GAMSGLLDPS QAMGPTLIGL AMGDAGGYKA SDMWGPKEDP AWQRNDPLLN VGKLIANNTR VWVYCGNGKP SDLGGNNLPA KFLEGFVRTS NIKFQDAYNA GGGHNGVFDF PDSGTHSWEY WGAQLNAMKP DLQRALGATP NTGPAPQGA