Recombinant E.coli mdh protein
Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction) . Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Recombinant E. coli mdh protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Malate dehydrogenase, ECK3225, JW3205
Expression System
E.coli
Antigen Species
E.coli
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
34.9 kDa (336aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Goward CR., et al. (1994) . Protein Sci. 3 (10) : 1883-8.; ; McAlister-Henn L., et al. (1988) . Trends Biochem. Sci. 13 (5) : 178-81.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_417703
Uniprot Accession Number
P61889
Species
E.coli
AA Sequence
Frequently Asked Questions
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