Recombinant E.coli mdh protein

Malate dehydrogenase (mdh) belongs to the LDH/MDH superfamily and MDH type 1 family. This enzyme catalyzes the conversion of malate into oxaloacetate (using NAD+) and vice versa (this is a reversible reaction) . Malate dehydrogenase is also involved in gluconeogenesis, the synthesis of glucose from smaller molecules. Recombinant E. coli mdh protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 1000 units/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Product Name Alternative

Malate dehydrogenase, ECK3225, JW3205

Expression System

E.coli

Antigen Species

E.coli

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 1000unit/mg, and is defined as the amount of enzyme that cleaves 1umole of oxalacetate and beta-NADH to L-malate and beta-NAD per minute at pH8.0 at 37C.

Molecular Weight

34.9 kDa (336aa) confirmed by MALDI-TOF

Additionnal Information

Mdh, malate dehydrogenase, ECK3225, JW3205, ATGP1487-10 µg, ATGP1487-20 µg, ATGP1487-50 µg, ATGP1487-100 µg, ATGP1487-250 µg, ATGP1487-500 µg, ATGP1487-1 mg, ATGP1487-10, ATGP1487-20, ATGP1487-50, ATGP1487-100, ATGP1487-250, ATGP1487-500, ATGP1487-1

References & Citations

Goward CR., et al. (1994) . Protein Sci. 3 (10) : 1883-8.; ; McAlister-Henn L., et al. (1988) . Trends Biochem. Sci. 13 (5) : 178-81.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_417703

Uniprot Accession Number

P61889

Species

E.coli

AA Sequence

MKVAVL GAAGGIGQAL ALLLKTQLPS GSELSLYDIA PVTPGVAVDL SHIPTAVKIK GFSGEDATPA LEGADVVLIS AGVARKPGMD RSDLFNVNAG IVKNLVQQVA KTCPKACIGI ITNPVNTTVA IAAEVLKKAG VYDKNKLFGV TTLDIIRSNT FVAELKGKQP GEVEVPVIGG HSGVTILPLL SQVPGVSFTE QEVADLTKRI QNAGTEVVEA KAGGGSATLS MGQAAARFGL SLVRALQGEQ GVVECAYVEG DGQYARFFSQ PLLLGKNGVE ERKSIGTLSA FEQNALEGML DTLKKDIALG EEFVNK