Recombinant Influenza A H1N1 Hemagglutinin/HA1 protein

HA1 (hemaggulutinin1) belongs to the influenza viruses hemagglutinin family. Influenza hemagglutinin (HA) or haemagglutinin is a type of hemagglutinin found on the surface of the influenza viruses. It is an antigenic glycoprotein. It is responsible for binding the virus to the cell that is being infected. HA protein has two functions. Firstly, it allows the recognition of target vertebrate cells, accomplished through the binding of these cells' sialic acid-containing receptors. Secondly, once bound it facilitates the entry of the viral genome into the target cells by causing the fusion of host endosomal membrane with the viral membrane. Recombinant Influenza A virus (A/ New York/3571/2009 (H1N1) ) HA1 protein, fused to His-tag at C-terminus, was expressed in insect cell using baculovirus expression system and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Hemagglutinin, Influenza A virus (A/New York/3571/2009 H1N1) hemagglutinin, HA

Expression System

Baculovirus

Antigen Species

Human, Influenza A-H1N1, Influenza A-H1N1 (human), Influenza A

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

37.8 kDa (339aa)

Additionnal Information

Hemagglutinin, ATGP1484-10 µg, ATGP1484-20 µg, ATGP1484-50 µg, ATGP1484-100 µg, ATGP1484-250 µg, ATGP1484-500 µg, ATGP1484-1 mg, ATGP1484-10, ATGP1484-20, ATGP1484-50, ATGP1484-100, ATGP1484-250, ATGP1484-500, ATGP1484-1

References & Citations

Russell RJ, et al. (2008) Proc. Natl. Acad. Sci. u.S.A. 105 (46) : 17736-41.; ; White JM, et al. (1997) Structural Biology of Viruses. Oxford university Press. pp. 80-104.

Other References

Yang E, et al. Protection of mice against pandemic H1N1 influenza virus challenge after immunization with baculovirus-expressed stabilizing peptide fusion hemagglutinin protein. J Microbiol Biotechnol. 2015 Feb;25 (2) :280-7.{https://pubmed.ncbi.nlm.nih.gov/25394603/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Scientific Category

Other

NCBI Accession Number

ACV04238.1

Uniprot Accession Number

C7RYS4

Species

Human, Influenza A-H1N1, Influenza A-H1N1 (human), Influenza A

AA Sequence

MDTLCIG YHANNSTDTV DTVLEKNVTV THSVNLLEDK HNGKLCKLRG VAPLHLGKCN IAGWILGNPE CESLSTASSW SYIVETSSSD NGTCYPGDFI DYEELREQLS SVSSFERFEI FPKTSSWPNH DSNKGVTAAC PHAGAKSFYK NLIWLVKKGN SYPKLSKSYI NDKGKEVLVL WGIHHPSTSA DQQSLYQNAD AYVFVGSSRY SKKFKPEIAI RPKVRDQEGR MNYYWTLVEP GDKITFEATG NLVVPRYAFA MERNAGSGII ISDTPVHDCN TTCQTPKGAI NTSLPFQNIH PITIGKCPKY VKSTKLRLAT GLRNVPSIQS R