Recombinant human IL-12 p40 protein

IL12P40 (interleukin 12 subunit beta), also known as natural killer cell stimulatory factor 2, or cytotoxic lymphocyte maturation factor 2, p40, belongs to the type I cytokine receptor family. This protein is a cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Interleukin 12 is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit encoded by this gene, and a 35 kD subunit encoded by IL12A. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. Recombinant human IL12P40 protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Interleukin 12B, Interleukin-12 beta chain, IL-12B, Cytotoxic lymphocyte maturation factor 40 kDa subunit, CLMF p40, IL-12 subunit p40, interleukin 12 p40, NK cell stimulatory factor chain 2, NKSF2, CLMF2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

35.8 kDa (315aa)

Additionnal Information

IL12B, IL12 p40, interleukin 12 subuit beta, CLMF, NKSF, CLMF2, NKSF2, IL-12B, ATGP1483-10 µg, ATGP1483-20 µg, ATGP1483-50 µg, ATGP1483-100 µg, ATGP1483-250 µg, ATGP1483-500 µg, ATGP1483-1 mg, ATGP1483-10, ATGP1483-20, ATGP1483-50, ATGP1483-100, ATGP1483-250, ATGP1483-500, ATGP1483-1

References & Citations

Oppmann B., et al (2000) Immunity 13:715-725 (2000) ; ; Presky et al. (1996) Proc. Natl. Acad. Sci. u.S.A. 93 (24) : 14002-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 100 mM NaCl, 0.1mM PMSF

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_002178

Uniprot Accession Number

P29460

Species

Human

AA Sequence

IWELKKD VYVVELDWYP DAPGEMVVLT CDTPEEDGIT WTLDQSSEVL GSGKTLTIQV KEFGDAGQYT CHKGGEVLSH SLLLLHKKED GIWSTDILKD QKEPKNKTFL RCEAKNYSGR FTCWWLTTIS TDLTFSVKSS RGSSDPQGVT CGAATLSAER VRGDNKEYEY SVECQEDSAC PAAEESLPIE VMVDAVHKLK YENYTSSFFI RDIIKPDPPK NLQLKPLKNS RQVEVSWEYP DTWSTPHSYF SLTFCVQVQG KSKREKKDRV FTDKTSATVI CRKNASISVR AQDRYYSSSW SEWASVPCS