Recombinant M. tuberculosis ESAT6 protein

ESAT6, the 6 kDa early secretory antigenic target of Mycobacterium tuberculosis, is a secretory protein and potent T cell antigen. ESAT6 has been shown to directly bind to the TLR2 receptor, inhibiting downstream signal transduction. Recently it was shown that ESAT6 is very potential as diagnostic for differentiation between the mycobacterial infection and BCG vaccination. Recombinant Mycobacterium tuberculosis CDC1551 ESAT6 protein, fused to His-tag at C-terminus, was expressed in Baculovirus and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Early secretory antigenic target 6 kDa, esxA, esaT6

Expression System

Baculovirus

Antigen Species

Mycobacterium tuberculosis

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by BCA assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

11 kDa (104aa)

Additionnal Information

ESAT6, ATGP1480-10 µg, ATGP1480-20 µg, ATGP1480-50 µg, ATGP1480-100 µg, ATGP1480-250 µg, ATGP1480-500 µg, ATGP1480-1 mg, ATGP1480-10, ATGP1480-20, ATGP1480-50, ATGP1480-100, ATGP1480-250, ATGP1480-500, ATGP1480-1, Early secretory antigenic target, 6 kDa

References & Citations

Pathak SK., et al. (2007) Natlmmunol. 8 (6) :610-8.; ; Matveeva NA., et al. (2011) Tsitol Genet. 45 (1) :11-7.

Other References

Satake Y, et al. Type-1 polarised dendritic cells are a potent immunogen against Mycobacterium tuberculosis. (Int J Tuberc Lung Dis. 2017) {https://pubmed.ncbi.nlm.nih.gov/28399967/}; ; Ouyang W, et al. Enabling electrical biomolecular detection in high ionic concentrations and enhancement of the detection limit thereof by coupling a nanofluidic crystal with reconfigurable ion concentration polarization. (Lab Chip. 2017) {https://pubmed.ncbi.nlm.nih.gov/28983543/}