Recombinant M. tuberculosis ESAT6 protein
ESAT6, the 6 kDa early secretory antigenic target of Mycobacterium tuberculosis, is a secretory protein and potent T cell antigen. ESAT6 has been shown to directly bind to the TLR2 receptor, inhibiting downstream signal transduction. Recently it was shown that ESAT6 is very potential as diagnostic for differentiation between the mycobacterial infection and BCG vaccination. Recombinant Mycobacterium tuberculosis CDC1551 ESAT6 protein, fused to His-tag at C-terminus, was expressed in Baculovirus and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Early secretory antigenic target 6 kDa, esxA, esaT6
Expression System
Baculovirus
Antigen Species
Mycobacterium tuberculosis
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by BCA assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Molecular Weight
11 kDa (104aa)
Additionnal Information
References & Citations
Pathak SK., et al. (2007) Natlmmunol. 8 (6) :610-8.; ; Matveeva NA., et al. (2011) Tsitol Genet. 45 (1) :11-7.
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol
Scientific Category
Other
NCBI Accession Number
NP_338543.1
Uniprot Accession Number
P9WNK6
Species
Mycobacterium tuberculosis
AA Sequence
MTEQQWN FAGIEAAASA IQGNVTSIHS LLDEGKQSLT KLAAAWGGSG SEAYQGVQQK WDATATELNN ALQNLARTIS EAGQAMASTE GNVTGMFA
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