Recombinant human BCAT1 protein

BCAT1, also known as branched chain amino-acid transaminase, cytosolic, catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. Recombinant human BCAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Branched chain amino-acid transaminase cytosolic, Branched chain amino-acid transaminase, cytosolic, BCATC, BCT1, ECA39, MECA39, PNAS121, PP18

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

45.4 kDa (409aa) confirmed by MALDI-TOF

Additionnal Information

BCAT1, Branched chain amino-acid transaminase cytosolic, Branched chain amino-acid transaminase, cytosolic, BCATC, BCT1, ECA39, MECA39, PNAS121, PP18, ATGP1479-10 µg, ATGP1479-20 µg, ATGP1479-50 µg, ATGP1479-100 µg, ATGP1479-250 µg, ATGP1479-500 µg, ATGP1479-1 mg, ATGP1479-10, ATGP1479-20, ATGP1479-50, ATGP1479-100, ATGP1479-250, ATGP1479-500, ATGP1479-1

References & Citations

Goto M., et al. (2005) J. Biol. Chem. 280:37246-37256; ; Schuldiner O., et al. (1996) Proc. Natl. Acad. Sci. u.S.A. 93:7143-7148

Other References

Günther J, et al. BAY-069, a Novel (Trifluoromethyl) pyrimidinedione-Based BCAT1/2 Inhibitor and Chemical Probe. (J Med Chem. 2022) {https://pubmed.ncbi.nlm.nih.gov/36261130/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Scientific Category

Metabolism

NCBI Accession Number

NP_005495

Uniprot Accession Number

P54687

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMKDCSNG CSAECTGEGG SKEVVGTFKA KDLIVTPATI LKEKPDPNNL VFGTVFTDHM LTVEWSSEFG WEKPHIKPLQ NLSLHPGSSA LHYAVELFEG LKAFRGVDNK IRLFQPNLNM DRMYRSAVRA TLPVFDKEEL LECIQQLVKL DQEWVPYSTS ASLYIRPTFI GTEPSLGVKK PTKALLFVLL SPVGPYFSSG TFNPVSLWAN PKYVRAWKGG TGDCKMGGNY GSSLFAQCEA VDNGCQQVLW LYGEDHQITE VGTMNLFLYW INEDGEEELA TPPLDGIILP GVTRRCILDL AHQWGEFKVS ERYLTMDDLT TALEGNRVRE MFGSGTACVV CPVSDILYKG ETIHIPTMEN GPKLASRILS KLTDIQYGRE ESDWTIVLS