Recombinant human EYA2 protein

EYA2, also known as eyes absent homolog 2, is a member of tyrosine phosphatase family that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph) . This protein promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Recombinant human EYA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Eyes absent homolog 2, EAB1, MGC10614

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

33.2 kDa (295aa) confirmed by MALDI-TOF

Additionnal Information

EYA2, Eyes absent homolog 2, EAB1, MGC10614, ATGP1478-10 µg, ATGP1478-20 µg, ATGP1478-50 µg, ATGP1478-100 µg, ATGP1478-250 µg, ATGP1478-500 µg, ATGP1478-1 mg, ATGP1478-10, ATGP1478-20, ATGP1478-50, ATGP1478-100, ATGP1478-250, ATGP1478-500, ATGP1478-1

References & Citations

Krishnan N., et al. (2009) J. Biol. Chem. 284:16066-16070; ; Fan X., et al. (2000) J. Biol. Chem. 275:32129-32134

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

AAH08803

Uniprot Accession Number

O00167

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MGSMERVFVW DLDETIIIFH SLLTGTFASR YGKDTTTSVR IGLMMEEMIF NLADTHLFFN DLEDCDQIHV DDVSSDDNGQ DLSTYNFSAD GFHSSAPAAN LCLGSGVHGG VDWMRKLAFR YRRVKEMYNT YKNNVGGLIG TPKRETWLQL RAELEALTDL WLTHSLKALN LINSRPNCVN VLVTTTQLIP ALAKVLLYGL GSVFPIENIY SATKTGKESC FERIMQRFGR KAVYVVIGDG VEEEQGAKKH NMPFWRISCH ADLEALRHAL ELEYL