Recombinant human MAGEA5 protein
MAGEA5, also known as melanoma-associated antigen 5, is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. This MAGEA gene encodes a protein that is C-terminally truncated compared to other family members, and this gene can be alternatively interpreted to be a pseudogene. Recombinant human MAGEA5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Melanoma-associated antigen 5, CT1.5, MAGE5, MAGEA4
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
15.6 kDa (148aa) confirmed by MALDI-TOF, (Molecular weight on SDS-PAGE will appear higher)
Additionnal Information
References & Citations
De Plaen E., et al. (1994) Immunogenetics. 40:360-369; ; The MGC Project Team. (2004) Genome Res. 14:2121-2127
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1mM PMSF
Scientific Category
Cancer
NCBI Accession Number
NP_066387
Uniprot Accession Number
P43359
Species
Human
AA Sequence
MSLEQK SQHCKPEEGL DTQEEALGLV GVQAATTEEQ EAVSSSSPLV PGTLGEVPAA GSPGPLKSPQ GASAIPTAID FTLWRQSIKG SSNQEEEGPS TSPDPESVFR AALSKKVADL IHFLLLKY
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