Recombinant mouse TNF-alpha protein

TNFa, also known as tumor necrosis factor, binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia. Recombinant mouse TNFa protein was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Expression System

E.coli

Antigen Species

Mouse

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

17.4 kDa (157aa) confirmed by MALDI-TOF

Additionnal Information

TNF-a, Tumor necrosis factor, DIF, TNF-alpha, TNFalpha, Tnfsf1a, TNFSF2, Cachectin, ATGP1475-10 µg, ATGP1475-20 µg, ATGP1475-50 µg, ATGP1475-100 µg, ATGP1475-250 µg, ATGP1475-500 µg, ATGP1475-1 mg, ATGP1475-10, ATGP1475-20, ATGP1475-50, ATGP1475-100, ATGP1475-250, ATGP1475-500, ATGP1475-1, ATGP1475-10 µg, ATGP1475-20 µg, ATGP1475-50 µg, ATGP1475-100 µg, ATGP1475-250 µg, ATGP1475-500 µg, ATGP1475-1 mg, ATGP1475-10, ATGP1475-20, ATGP1475-50, ATGP1475-100, ATGP1475-250, ATGP1475-500, ATGP1475-1

References & Citations

Shakhov A.N., et al. (1987) Bioorg. Khim. 13:701-705; ; Pennica D., et al. (1985) Proc. Natl. Acad. Sci. u.S.A. 82:6060-6064

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_038721

Uniprot Accession Number

P06804

Species

Mouse

AA Sequence

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL