Recombinant human N-Acetyl-D-Glucosamine Kinase/NAGK protein

NAGK (N-acetyl-D-glucosamine kinase) belongs to the eukaryotic-type N-acetylglucosamine kinase family. NAGK converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. It is a prominent salvage enzyme of amino sugar metabolism in mammals. NAGK has been shown to interact with STK16 and LNX1. Also it has ManNAc kinase activity. Recombinant human NAGK protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

N-acetyl-D-glucosamine kinase, GNK, HSA242910, GlcNAc kinase

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

39.8 kDa (367aa) confirmed by MALDI-TOF

Additionnal Information

N-Acetyl-D-Glucosamine Kinase, NAGK, N-acetyl-D-glucosamine kinase, GNK, HSA242910, GlcNAc kinase, ATGP1474-10 µg, ATGP1474-20 µg, ATGP1474-50 µg, ATGP1474-100 µg, ATGP1474-250 µg, ATGP1474-500 µg, ATGP1474-1 mg, ATGP1474-10, ATGP1474-20, ATGP1474-50, ATGP1474-100, ATGP1474-250, ATGP1474-500, ATGP1474-1

References & Citations

Hinderlich S, et al. (2000) Eur J Biochem 267 (11) : 3301-8.; ; Weihofen W.A., et al. (2006) J. Mol. Biol. 364:388-399

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_060037

Uniprot Accession Number

Q9UJ70

Species

Human

AA Sequence

MAAIYGG VEGGGTRSEV LLVSEDGKIL AEADGLSTNH WLIGTDKCVE RINEMVNRAK RKAGVDPLVP LRSLGLSLSG GDQEDAGRIL IEELRDRFPY LSESYLITTD AAGSIATATP DGGVVLISGT GSNCRLINPD GSESGCGGWG HMMGDEGSAY WIAHQAVKIV FDSIDNLEAA PHDIGYVKQA MFHYFQVPDR LGILTHLYRD FDKCRFAGFC RKIAEGAQQG DPLSRYIFRK AGEMLGRHIV AVLPEIDPVL FQGKIGLPIL CVGSVWKSWE LLKEGFLLAL TQGREIQAQN FFSSFTLMKL RHSSALGGAS LGARHIGHLL PMDYSANAIA FYSYTFS