Recombinant human PLDN protein

PLDN (Pallidin) may play a role in intracellular vesicle trafficking. It interacts with Syntaxin 13 which mediates intracellular membrane fusion. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. This protein involved in the development of lysosome-related organelles, such as melanosomes and platelet-dense granules. PLDN has been shown to interact with BLOC1S1, STX12, Dysbindin, CNO, BLOC1S2, MuTED and SNAPAP. Recombinant human PLDN protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Pallidin, HPS9, PA, PALLID

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

21.9 kDa (192aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

PLDN, pallidin, HPS9, PA, PALLID, ATGP1472-10 µg, ATGP1472-20 µg, ATGP1472-50 µg, ATGP1472-100 µg, ATGP1472-250 µg, ATGP1472-500 µg, ATGP1472-1 mg, ATGP1472-10, ATGP1472-20, ATGP1472-50, ATGP1472-100, ATGP1472-250, ATGP1472-500, ATGP1472-1

References & Citations

Huang L, et al. (1999) . Nat Genet 23 (3) : 329-32.; ; Moriyama K., et al. (2002) Traffic 3:666-677

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100 mM NaCl

Scientific Category

Signal Transduction

NCBI Accession Number

NP_036520

Uniprot Accession Number

Q9UL45

Species

Human

AA Sequence

MSVPGPSSPD GALTRPPYCL EAGEPTPGLS DTSPDEGLIE DLTIEDKAVE QLAEGLLSHY LPDLQRSKQA LQELTQNQVV LLDTLEQEIS KFKECHSMLD INALFAEAKH YHAKLVNIRK EMLMLHEKTS KLKKRALKLQ QKRQKEELER EQQREKEFER EKQLTARPAK RM