Recombinant human ADH5 protein
ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. Recombinant human ADH5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Alcohol dehydrogenase class-3, ADHX, FDH
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
42.3 kDa (398aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Zhang, X. et al. (2010) Carcinogenesis 31 (12), 2118-2123; ; Shimada, M. et al. (2010) Hum. Genet. 128 (4), 433-441
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol 0.1M Nacl, 1mM DTT
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000662
Uniprot Accession Number
P11766
Species
Human
AA Sequence
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