Recombinant human ARHGDIB protein

Members of the Rho (or ARH) protein family and other Ras-related small GTP-binding proteins are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs) . The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases. Recombinant human ARHGDIB protein, fused to GST-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Rho GDP-dissociation inhibitor 2, D4, GDIA2, GDID4, Ly-GDI, LYGDI, RAP1GN1; RhoGDI2

Expression System

E.coli

Antigen Species

Human

Tag

GST-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

49.4 kDa (428aa)

Additionnal Information

ARHGDIB, Rho GDP-dissociation inhibitor 2, D4, GDIA2, GDID4, Ly-GDI, LYGDI, RAP1GN1; RhoGDI2, ATGP1470-10 µg, ATGP1470-20 µg, ATGP1470-50 µg, ATGP1470-100 µg, ATGP1470-250 µg, ATGP1470-500 µg, ATGP1470-1 mg, ATGP1470-10, ATGP1470-20, ATGP1470-50, ATGP1470-100, ATGP1470-250, ATGP1470-500, ATGP1470-1

References & Citations

Skalnikova, H. et al. (2011) J. Proteome Res. 10 (2), 404-415; ; Mehta, P. et al. (2011) PLoS ONE 6 (6), E21175

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001166

Uniprot Accession Number

P52566

Species

Human

AA Sequence

MTE KAPEPHVEED DDDELDSKLN YKPPPQKSLK ELQEMDKDDE SLIKYKKTLL GDGPVVTDPK APNVVVTRLT LVCESAPGPI TMDLTGDLEA LKKETIVLKE GSEYRVKIHF KVNRDIVSGL KYVQHTYRTG VKVDKATFMV GSYGPRPEEY EFLTPVEEAP KGMLARGTYH NKSFFTDDDK QDHLSWEWNL SIKKEWTE