Recombinant mouse VPS29 protein
Vacuolar protein sorting 29, also known as VPS29, belongs to a group of genes coding for vacuolar protein sorting (VPS) proteins that, when functionally impaired, disrupt the efficient delivery of vacuolar hydrolases. It is a late Golgi transmembrane protein that acts as the sorting receptor for soluble vacuolar hydrolases, from the prevacuolar endosome back to the Golgi. Also, VPS29 may be involved in the formation of the inner shell of the retromer coat for retrograde vesicles leaving the prevacuolar compartment. Recombinant Mouse VPS29 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
VPS29 retromer complex component, Vacuolar protein sorting-associated protein 29, Vesicle protein sorting 29, PEP11, DC7, DC15
Expression System
E.coli
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
23.2 kDa (207aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Edgar AJ., et al. (2000) Biochem Biophys Res Commun. 277 (3) :622-30.; ; Wanq D., et al. (2005) J Biol Chem. 280 (24) :22962-7.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Transportation
NCBI Accession Number
NP_062754
Uniprot Accession Number
Q9QZ88
Species
Mouse
AA Sequence
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