Recombinant human CCDC101/SGF29 protein
Coiled-coil domain containing 101 (CCDC101), also known as SGF29, has been identified as a subunit of the SAGA (Spt-Ada-Gcn5 acetyltransferase) histone acetyltransferase complex in Saccharomyces cerevisiae, which is conserved from yeast to humans. Recombinant human CCDC101 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Coiled-coil domain containing 101, SGF29, STAF36
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
35.4 kDa (313aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Bian C., et al. (2011) EMBO J. 30 (14) :2829-42; ; LiJ., et al. (2010) Acta Crystallogr Sect F Struct Biol Cryst Commun. 66:902-4.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_612423
Uniprot Accession Number
Q96ES7
Species
Human
AA Sequence
Frequently Asked Questions
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