Recombinant human CCDC101/SGF29 protein

Coiled-coil domain containing 101 (CCDC101), also known as SGF29, has been identified as a subunit of the SAGA (Spt-Ada-Gcn5 acetyltransferase) histone acetyltransferase complex in Saccharomyces cerevisiae, which is conserved from yeast to humans. Recombinant human CCDC101 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Coiled-coil domain containing 101, SGF29, STAF36

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

35.4 kDa (313aa) confirmed by MALDI-TOF

Additionnal Information

CCDC101, Coiled-coil domain containing 101, SGF29, STAF36, ATGP1462-10 µg, ATGP1462-20 µg, ATGP1462-50 µg, ATGP1462-100 µg, ATGP1462-250 µg, ATGP1462-500 µg, ATGP1462-1 mg, ATGP1462-10, ATGP1462-20, ATGP1462-50, ATGP1462-100, ATGP1462-250, ATGP1462-500, ATGP1462-1

References & Citations

Bian C., et al. (2011) EMBO J. 30 (14) :2829-42; ; LiJ., et al. (2010) Acta Crystallogr Sect F Struct Biol Cryst Commun. 66:902-4.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_612423

Uniprot Accession Number

Q96ES7

Species

Human

AA Sequence

MALVSADSRI AELLTELHQL IKQTQEERSR SEHNLVNIQK THERMQTENK ISPYYRTKLR GLYTTAKADA EAECNILRKA LDKIAEIKSL LEERRIAAKI AGLYNDSEPP RKTMRRGVLM TLLQQSAMTL PLWIGKPGDK PPPLCGAIPA SGDYVARPGD KVAARVKAVD GDEQWILAEV VSYSHATNKY EVDDIDEEGK ERHTLSRRRV IPLPQWKANP ETDPEALFQK EQLVLALYPQ TTCFYRALIH APPQRPQDDY SVLFEDTSYA DGYSPPLNVA QRYVVACKEP KKK