Recombinant human CBX5 protein

Chromobox homolog 5, also known as CBX5, is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. CBX5 is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. It has a pseudogene located on chromosome 3. Also, The CBX5 proteins reassociate with chromatin at the end of mitosis, as Histone H3 is dephosphorylated. Recombinant human CBX5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Chromobox homolog 5, HP1, HP1A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

24.8 kDa (215aa) confirmed by MALDI-TOF

Additionnal Information

CBX5, ATGP1461-10 µg, ATGP1461-20 µg, ATGP1461-50 µg, ATGP1461-100 µg, ATGP1461-250 µg, ATGP1461-500 µg, ATGP1461-1 mg, ATGP1461-10, ATGP1461-20, ATGP1461-50, ATGP1461-100, ATGP1461-250, ATGP1461-500, ATGP1461-1

References & Citations

Koike N., et al. (2000) FEBS Lett. 467:17-21.; ; Verreault A., et al. (1996) Cell. 87:95-104

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_001120794

Uniprot Accession Number

P45973

Species

Human

AA Sequence

MGKKTK RTADSSSSED EEEYVVEKVL DRRVVKGQVE YLLKWKGFSE EHNTWEPEKN LDCPELISEF MKKYKKMKEG ENNKPREKSE SNKRKSNFSN SADDIKSKKK REQSNDIARG FERGLEPEKI IGATDSCGDL MFLMKWKDTD EADLVLAKEA NVKCPQIVIA FYEERLTWHA YPEDAENKEK ETAKS