Recombinant human CARD18 protein
Caspase recruitment domain-containing protein 18, also known as CARD18, is a member of the death-domain-fold superfamily that is induced by proinflammatory stimuli. It consists essentially only of a CARD, inhibits generation of IL-1b by interacting with caspase-1 and preventing association with RIP 2. The association of CARD18 with caspase-1 is facilitated by the charge-charge interactions between the prodomain of caspase-1 and the surface charge of CARD18. Recombinant human CARD18 proetin, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Caspase recruitment domain-containing protein 18, ICEBERG, pseudo-ICE, uNQ5804
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
12.3 kDa (110aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Humke E W., et al. (2000) Cell. 103:99-111.; ; Watson R W., et al. (1998) J Immunol. 161:957-962
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,10% glycerol, 0.1M NaCl
Scientific Category
Apoptosis
NCBI Accession Number
NP_067546
Uniprot Accession Number
P57730
Species
Human
AA Sequence
MGSSHHHHHH SSGLVPRGSH MADQLLRKKR RIFIHSVGAG TINALLDCLL EDEVISQEDM NKVRDENDTV MDKARVLIDL VTGKGPKSCC KFIKHLCEED PQLASKMGLH
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