Recombinant human EXOSC5 protein

EXOSC5, also known as exosome component 5, belongs to the RNase PH family. EXOSC5 has been shown to interact with Exosome component 8 and Exosome component 1. It is Non-catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. It highly expressed in a variety of hematopoietic and epithelial tumor cell lines, but not in normal hematopoietic tissues or other normal tissue, with the exception of testis. Recombinant human EXOSC5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Exosome complex component RRP46, hRrp46p, p12B, RRP41B, RRP46, Rrp46p

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

27.5 kDa (256aa) confirmed by MALDI-TOF

Additionnal Information

EXOSC5, Exosome complex component RRP46, hRrp46p, p12B, RRP41B, RRP46, Rrp46p, ATGP1458-10 µg, ATGP1458-20 µg, ATGP1458-50 µg, ATGP1458-100 µg, ATGP1458-250 µg, ATGP1458-500 µg, ATGP1458-1 mg, ATGP1458-10, ATGP1458-20, ATGP1458-50, ATGP1458-100, ATGP1458-250, ATGP1458-500, ATGP1458-1

References & Citations

Mukherjee D., et al. (2002) EMBO J. 21:165-174; ; Basu u., et al. (2011) Cell 144:353-363

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 2mM DTT, 30% glycerol, 200 mM NaCl

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_064543.3

Uniprot Accession Number

Q9NQT4

Species

Human

AA Sequence

MEEETHTDA KIRAENGTGS SPRGPGCSLR HFACEQNLLS RPDGSASFLQ GDTSVLAGVY GPAEVKVSKE IFNKATLEVI LRPKIGLPGV AEKSRERLIR NTCEAVVLGT LHPRTSITVV LQVVSDAGSL LACCLNAACM ALVDAGVPMR ALFCGVACAL DSDGTLVLDP TSKQEKEARA VLTFALDSVE RKLLMSSTKG LYSDTELQQC LAAAQAASQH VFRFYRESLQ RRYSKS