Recombinant human DARS protein
DARS, also known as aspartyl-tRNA synthetase, catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. Recombinant human DARS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
Product Specifications
Product Name Alternative
Aspartyl-tRNA synthetase
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
59.2 kDa (521aa)
Additionnal Information
References & Citations
Guzzo C.M., et al. (2008) Biochem. Biophys. Res. Commun. 365:718-723; ; Imami K., et al. (2001) Anal. Sci. 24:161-166;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT
Scientific Category
Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_001340
Uniprot Accession Number
P14868
Species
Human
AA Sequence
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