Recombinant human STYX protein

STYX, also known as serine/threonine/tyrosine-interacting protein, belongs to the protein-tyrosine phosphatase family. It contains a Gly residue instead of a conserved Cys residue in the dsPTPase catalytic loop which renders it catalytically inactive as a phosphatase. The binding pocket is however sufficiently preserved to bind phosphorylated substrates, and maybe protect them from phosphatases. It seems to play a role in spermiogenesis. Recombinant human STYX protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Product Specifications

Product Name Alternative

Serine/threonine/tyrosine-interacting protein, FLJ42934

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

25.0 kDa (221aa) confirmed by MALDI-TOF

Additionnal Information

STYX, Serine/threonine/tyrosine-interacting protein, FLJ42934, ATGP1452-10 µg, ATGP1452-20 µg, ATGP1452-50 µg, ATGP1452-100 µg, ATGP1452-250 µg, ATGP1452-500 µg, ATGP1452-1 mg, ATGP1452-10, ATGP1452-20, ATGP1452-50, ATGP1452-100, ATGP1452-250, ATGP1452-500, ATGP1452-1

References & Citations

Wishart MJ. et al. (1995) J. Biol. Chem. 270:26782-26785.; ; Wishart MJ. et al. (2002) Proc Natl Acad Sci u S A. 99:2112-2117.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_660294

Uniprot Accession Number

Q8WUJ0

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH SHMRREMQEI LPGLFLGPYS SAMKSKLPVL QKHGITHIIC IRQNIEANFI KPNFQQLFRY LVLDIADNPV ENIIRFFPMT KEFIDGSLQM GGKVLVHGNA GISRSAAFVI AYIMETFGMK YRDAFAYVQE RRFCINPNAG FVHQLQEYEA IYLAKLTIQM MSPLQIERSL SVHSGTTGSL KRTHEEEDDF GTMQVATAQN G